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Laboratory Applications in Microbiology: A Case Study Approach

ISBN10: 1260418774 | ISBN13: 9781260418774

Laboratory Applications in Microbiology: A Case Study Approach
ISBN10: 1260418774
ISBN13: 9781260418774
By Barry Chess

* The estimated amount of time this product will be on the market is based on a number of factors, including faculty input to instructional design and the prior revision cycle and updates to academic research-which typically results in a revision cycle ranging from every two to four years for this product. Pricing subject to change at any time.

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Laboratory Applications in Microbiology: A Case Study Approach has been designed to help create accomplished microbiologists. Case studies illustrate the applicability of skills in the microbiology lab, while still holding the attention of every student who has ever said “do we have to know this?”


Each exercise has been structured from the bottom up, scaffolding knowledge and relying on metacognition to ensure students understand the goals of an exercise, anticipate errors, acquire the skills needed for success, and eventually master the topic at hand.;/div>

1 Safety Considerations in the Microbiology Laboratory
2 Microscopy and Measurement of Microscopic Specimens
3 A Survey of Protists
4 A Survey of Fungi
5 A Survey of Parasitic Worms
6 Ubiquity of Microorganisms
7 Aseptic and Pure Culture Techniques
8 Simple Staining, Negative Staining, and Gram Staining
9 Capsular Staining
10 Endospore Staining
11 Acid-Fast Staining
12 Viable Plate Count
13 Cultivation of Anaerobes
14 Temperature Effects on Bacterial Growth and Survival
15 pH and Microbial Growth
16 Effects of Osmotic Pressure on Bacterial Growth
17 Lethal Effects of Ultraviolet Light
18 Evaluation of Disinfectants
19 Effectiveness of Hand Scrubbing
20 Antimicrobic Sensitivity Testing: Kirby-Bauer, Tube Dilution, and ETEST(C) Methods
21 Simulated Epidemic
22 Morbidity and Mortality Weekly Report
23 Bacterial Transformation
24 The Ames Test
25 DNA Extraction from Bacterial Cells
26 DNA Profiling
27 Blood Typing
28 Rapid Identification of Staphylococcus aureus Using Latex Agglutination Testing
29 Slide Agglutination
30 Enzyme-Linked Immunosorbent Assay (ELISA)
31 Biofilm Culture and Examination
32 Measures of Water Quality: Most Probable Number Procedure
33 Measures of Water Quality: Membrane Filtration Method
34 Measures of Milk Quality: Methylene Blue Reductase Test
35 Bacterial Counts of Food
36 Isolation and Identification of Staphylococci
37 Isolation and Identification of Streptococci
38 Epidemiology of Gastrointestinal Illness: Differentiation of Enterobacterlaceae
39 Differential White Blood Cell Count
40 Identification of Bacterial Unknowns
41 Colony Morphology
42 Growth in Solid and Liquid Media
43 Motilty Methods: Wet Mount and Hanging Drop
44 Flagella Stain
45 Streak-Plate Isolation
46 Loop Dilution
47 Spread-Plate
48 Fluid Thioglycollate Medium
49 CHROMagar Orientation Medium
50 Mannitol Salt Agar
51 MacConkey Agar
52 Desoxycholate Agar
53 Endo Agar
54 Eosin Methylene Blue Agar
55 Hektoen Enteric Agar
56 Xylose Lysine Desoxycholate Agar
57 Blood Agar
58 Motility Medium
59 SIM Medium
60 Kligler's Iron Agar
61 Triple Sugar Iron Agar
62 Lysine Iron Agar
63 Litmus Milk
64 Oxidation-Fermentation Test
65 Phenol Red Carbohydrate Broth
66 Purple Carbohydrate Broth
67 Methyl Red and Voges-ProskauerTests
68 Catalase Test
69 Oxidase Test
70 Nitrate Reduction Test
71 Coagulase Test
72 Citrate Test
73 Malonate Test
74 Amino Acid Decarboxylation Test
75 Phenylalanine Deaminase Test
76 Bile Esculin Test
77 Starch Hydrolysis
78 ONPG Test
79 Urease Test
80 Casease Test
81 Gelatinase Test
82 DNase Test
83 Lipase Test
84 CAMP Test
85 PYR Test
86 API 20E System
87 EnteroPluri-Test System
88 Antibiotic Disk Sensitivity Tests
89 B-Lactamase Test
90 Viable Plate Count
91 Direct Cell Count

About the Author

Barry Chess

Barry Chess has taught microbiology at Pasadena City College for more than twenty years. Prior to that, while studying at the California State University and the University of California, he conducted research into the expression of genes involved in the development of muscle and bone.

At PCC, beyond his usual presence in the microbiology laboratory and lecture hall, Barry has taught majors and non-majors biology, developed a course in human genetics, helped to found a biotechnology program on campus, and regularly supervises students completing independent research projects in the life sciences.Over the past several years, his interests have focused on innovative methods of teaching that lead to greater student success. He has written and reviewed cases for the National Center for Case Study Teaching in Science and contributed to the book Science Stories You Can Count On: 51 Case Studies with Quantitative Reasoning in Biology. Barry has presented papers and talks on the effective use of case studies in the classroom, the use of digital tools to enhance learning, and for several years served as a scientific advisor for the American Film Institute. In addition to Laboratory Applications in Microbiology, Barry is coauthor of the lecture text Foundations in Microbiology, now in its tenth edition. He is a member of the American Association for the Advancement of Science, and the American Society for Microbiology, and the Skeptics Society. Barry was profiled in the book, What Scientists Actually Do, where he was illustrated as a young girl with pigtails, about to stick a fork into an electrical outlet.

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