Microbiology

1st Lab Tutorial:

• Virtual Labs Tutorial

Applying the Scientific Method:

• Pillbug Preference

• Ubiquity of Microorganisms

Aseptic Technique:

• Broth Culture to Sterile Agar Plate

• Broth Culture to Sterile Broth

• Slant Culture to Sterile Agar Slant

Bacterial Genetics:

• DNA Profiling

• Polymerase Chain Reaction (PCR)

• Bacterial Transformation

Basic Chemistry:

•  Chemistry for Biology

• Density of a Cube

• Properties of Water

• Synthesis of Calcium Carbonate

• Titration of Vinegar

Blood:

• Differential White Blood Cell Count

• Hematocrit

• Hemoglobin Content

• Blood Typing

Biochemical Testing (Unknown ID Tests)

• Antimicrobial Testing for Gram-Positive Cocci Identification

• Blood agar hemolysis test

• CAMP test

• Catalase test

• Citrate test

• Coagulase test

• Phenol red test (glucose fermentation)

• Triple sugar iron (TSI) agar

• Indole test

• MacConkey agar (lactose fermentation)

• Mannitol salt agar

• Methyl red test

• Voges-Proskauer test

• SIM medium

• Nitrate reduction test

• Oxidase test

• Urease Test

Cardiovascular Physiology:

• Pulse Rate

• Blood Pressure

• Electrocardiography

• Heart Auscultation

Cell Division:

• Examining Meiosis

• Examining Mitosis

Cell Structure:

• Examining Plant & Animal Cells

• Prokaryotic and Eukaryotic Cell Size, Shape, and Function

Cellular Respiration:

• Yeast Fermentation

• Measuring Energy Production in Plants

Chemical Composition of Cells:

• Test for Starch

• Test for Sugars

• Digestion of Starch

• Emulsification of Lipids

• Test for Fat

• Test for Proteins

Control of Microbial Growth:

• Effect of Ultraviolet Light

• Effect of Antiseptics and Disinfectants

• Antimicrobial Sensitivity Testing (Kirby-Bauer Method)

Diffusion:

• Effect of Concentration on the Rate of Diffusion in a Semisolid

• Effect of Density of Media on the Rate of Diffusion

• Effect of Molecular Weight on the Rate of Diffusion in Air

• Diffusion Across a Selectively Permeable Membrane

Digestive System:

• Enzymes & Digestion

Dissections:

• Dissection Tutorial for Animals and Plants

• Earthworm (invertebrate)

• Mussel (invertebrate)

• Perch (vertebrate)

• Crayfish (invertebrate)

• Shark (Vertebrate)

• Sea Star (invertebrate)

• Fetal Pig (vertebrate)

• Frog (vertebrate)

DNA Biology and Technology:

• Enzyme-Linked Immunosorbent Assay (ELISA) Testing (New)

• Isolation of DNA

• Gel Electrophoresis

• DNA/RNA Structure

• Rapid Diagnostic Testing

• Transcription, Translation, and Mutation

Electromyography:

• Motor Unit Recruitment

• Time to Fatigue

Endocrine System:

• Influence of Thyroid Hormone on Temperature Regulation

• Effects of Blood Glucose Level

Environmental Studies:

• Climate change

• Water Quality

Evidence of Evolution:

• Fossils and Comparative Anatomy

• Molecular Evidence

• Phylogeny and Cladistics

Eye and Vision:

• Eye Dissection

• Accommodation of the Lens

• Astigmatism Test

• Blind Spot Demonstration

• Color Vision Test

• Convergence Reflex Test

• Pupillary Reflex Test

• Visual Acuity Test

How Enzymes Work:

• Effect of Temperature

• Enzyme Activity

• Effect of pH

• Effect of Concentration

Human Genetics:

• Chromosomal Inheritance During Meiosis

• Genetic Inheritance

Isolation Methods:

• Quadrant Streak Plate Method

• Pour Plating Method

• Subculturing of Bacteria

• Quantitative Dilution of Bacteria

• Quantification by Colony Counting

• Optical Density

Lab Safety:

• Hand Washing Procedure

• Personal Safety

Mendelian Genetics:

• Monohybrid Plant Cross

• Fruit Fly Characteristics

• Monohybrid Fruit Fly Cross

• Dihybrid Plant Cross

• Dihybrid Fruit Fly Cross

• X-Linked Fruit Fly Cross

Metric Measurement:

• Length

• Weight

• Volume

• Temperature

Microbial Growth:

• Oxygen Requirements & Anaerobic Jar

• Effects of Osmotic Pressure

• Effects of Temperature

• Oxygen Requirements and Fluid Thioglycolate Medium Tubes

• Effects of pH

Microscopy:

• Operation of a Brightfield Microscope

• Oil Immersion

• Pond Water Wet Mount

• Plant Cells (Onion Epidermal Cells)

• Animal Cells

• Diversity of Microorganisms

• Epithelial Tissue Histology

• Connective Tissue Histology

• Muscle Tissue Histology

• Nervous Tissue Histology

• Euglena Wet Mount