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LooseLeaf Benson's Microbiological Applications Laboratory Manual--Concise Version, 14th Edition
ISBN10: 1259705234 | ISBN13: 9781259705236
© 2017
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Benson's Microbiological Applications has been the "gold standard" of microbiology laboratory manuals for over 35 years. This manual has a number of attractive features that resulted in its adoption in universities, colleges, and community colleges. These features include user-friendly diagrams that students can easily follow, clear instructions, and an excellent array of reliable exercises suitable for beginning or advanced microbiology courses. In revising the lab manual for the fourteenth edition, we have tried to maintain the proven strengths of the manual and further enhance it. We have updated the introductory material of the fungi, protozoa, and algae to reflect changes in scientific information. Finally, the names of microorganisms used by the American Type Culture Collection. This is important for those users who rely on the ATCC for a source of cultures.
Part 1 Microscopy
1 Brightfield Microscopy
2 Darkfield Microscopy
3 Phase-Contrast Microscopy
4 Microscopic Measurements
Part 2 Survey of Microorganisms
5 Microbiology of Pond Water - Protists, Algae, and Cyanobacteria
6 Ubiquity of Bacteria
7 The Fungi: Molds and Yeasts
Part 3 Manipulation of Microorganisms
8 Aseptic Technique
9 Pure Culture Techniques
Part 4 Staining and Observations of Microorganisms
10 Smear Preparation
11 Simple Staining
12 Negative Staining
13 Capsular Staining
14 Gram Staining
15 Spore Staining: Two Methods
16 Acid-Fast Staining: Kinyoun Method
17 Motility Determination
Part 5 Culture Methods
18 Culture Media Preparation
19 Enumeration of Bacteria: The Standard Plate Count
20 Slide Culture: Fungi
Part 6 Bacterial Viruses
21 Determination of a Bacteriophage Titer
22 Isolation of Phage from Flies
23 Phage Typing
Part 7 Environmental Influences and Control of Microbial Growth
24 Effects of Oxygen on Growth
25 Temperature: Effects on Growth
26 pH and Microbial Growth
27 Water Activity and Osmotic Pressure
28 Ultraviolet Light: Lethal Effects
29 The Effects of Lysozyme on Bacterial Cells
30 Evaluation of Alcohol: Its Effectiveness as an Antiseptic
31 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method
32 Evaluation of Antiseptics: The Filter Paper Disk Method
33 Effectiveness of Hand Scrubbing
Part 8 Identification of Unknown Bacteria
34 Morphological Study of An Unknown Bacterium
35 Cultural Characteristics
36 Physiological Characteristics: Oxidation and Fermentation Stet Reactions
37 Physiological Characteristics: Hydrolytic and Degradative Stet Reactions
38 Physiological Characteristics: Multiple Test Media
39 Use of Bergey’s Manual
Part 9 Miniaturized Multitest Systems
40 Enterobacteriaceae Identification: The API 20E System
41 Enterobacteriaceae Identification: The Entero-Pluri Test
42 Staphylococcus Identification: The API Staph System
Part 10 Applied Microbiology
43 Bacterial Food Counts
44 Bacteriological Examination of Water: Most Probable Number Determination
45 Bacteriological Examination of Water: The Membrane Filter Method
46 Reductase Test
47 Temperature: Lethal Effects
48 Microbial Spoilage of Canned Food
49 Microbiology of Alcohol Fermentation
Part 11 Bacterial Genetics and Biotechnology
50 Polymerase Chain
51 Bacterial Transformation
Part 12 Immunology and Serology
52 The Staphylococci: Isolation and Identification
53 The Streptococci and Enterococci: Isolation and Identification
54 Gram-Negative Intestinal Pathogens
55 A Synthetic Epidemic
Part 13 Immunology and Serology
56 Slide Agglutination Test: Serological Typing
57 Slide Agglutination Test for S. aureus
58 Slide Agglutination Test for Streptococcus
59 Enzyme-Linked Immunosorbent Assay (ELISA)
60 Blood Grouping
Appendices
Appendix A Tables
Appendix B Indicators, Stains, Reagents
Appendix C Media
Appendix D Identification Charts
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