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Benson's Microbiological Applications Laboratory Manual--Complete Version, 14th Edition
ISBN10: 125991979X | ISBN13: 9781259919794
© 2017
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Benson's Microbiological Applications has been the "gold standard" of microbiology laboratory manuals for over 35 years. This manual has a number of attractive features that resulted in its adoption in universities, colleges, and community colleges. These features include user-friendly diagrams that students can easily follow, clear instructions, and an excellent array of reliable exercises suitable for beginning or advanced microbiology courses. In revising the lab manual for the fourteenth edition, we have tried to maintain the proven strengths of the manual and further enhance it. We have updated the introductory material of the fungi, protozoa, and algae to reflect changes in scientific information. Finally, the names of microorganisms used by the American Type Culture Collection. This is important for those users who rely on the ATCC for a source of cultures.
Part 1 Microscopy
1 Brightfield Microscopy
2 Darkfield Microscopy
3 Phase-Contrast Microscopy
4 Fluorescence Microscopy
5 Microscopic Measurements
Part 2 Survey of Microorganisms
6 Microbiology of Pond Water - Protists, Algae, and Cyanobacteria
7 Ubiquity of Bacteria
8 The Fungi: Molds and Yeasts
Part 3 Manipulation of Microorganisms
9 Aseptic Technique
10 Pure Culture Techniques
Part 4 Staining and Observations of Microorganisms
11 Smear Preparation
12 Simple Staining
13 Negative Staining
14 Capsular Staining
15 Gram Staining
16 Spore Staining: Two Methods
17 Acid-Fast Staining: Kinyoun Method
18 Motility Determination
Part 5 Culture Methods
19 Culture Media Preparation
20 Preparation of Stock Cultures
21 Enumeration of Bacteria: The Standard Plate Count
22 Slide Culture: Fungi
Part 6 Bacterial Viruses
23 Determination of a Bacteriophage Titer
24 A One-Step Bacteriophage Growth Curve
25 Isolation of Phage from Flies
26 Phage Typing
Part 7 Environmental Influences and Control of Microbial Growth
27 Effects of Oxygen on Growth
28 Temperature: Effects on Growth
29 pH and Microbial Growth
30 Water Activity and Osmotic Pressure
31 Ultraviolet Light: Lethal Effects
32 The Effects of Lysozyme on Bacterial Cells
33 Evaluation of Alcohol: Its Effectiveness as an Antiseptic
34 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method
35 Evaluation of Antiseptics: The Filter Paper Disk Method
36 Effectiveness of Hand Scrubbing
Part 8 Identification of Unknown Bacteria
37 Morphological Study of An Unknown Bacterium
38 Cultural Characteristics
39 Physiological Characteristics: Oxidation and Fermentation Stet Reactions
40 Physiological Characteristics: Hydrolytic and Degradative Stet Reactions
41 Physiological Characteristics: Multiple Test Media
42 Use of Bergey’s Manual
Part 9 Miniaturized Multitest Systems
43 Enterobacteriaceae Identification: The API 20E System
44 Enterobacteriaceae Identification: Entero-Pluri Test
45 Staphylococcus Identification: The API Staph System
Part 10 Diversity and Environmental Microbiology
46 Isolation of an Antibiotic Producer: The Streptomyces
47 Nitrogen Cycle: Ammonification
48 Symbiotic Nitrogen Fixation: Rhizobium
49 Free-Living Nitrogen Fixation: Azotobacter
50 Denitrification: Paracoccus denitrificans
51 The Winogradsky Column
52 Purple Nonsulfur Photosynthetic Bacteria
53 Sulfate Reducing Bacteria: Desulfovibrio
54 Bacterial Commensalism
55 Bacterial Synergism
56 Microbial Antagonism
Part 11 Applied Microbiology
57 Bacterial Food Counts
58 Bacteriological Examination of Water: Most Probable Number Determination
59 Bacteriological Examination of Water: The Membrane Filter Method
60 Reductase Test
61 Temperature: Lethal Effects
62 Microbial Spoilage of Canned Food
63 Microbiology of Alcohol Fermentation
Part 12 Bacterial Genetics and Biotechnology
64 Understanding Antibiotic Resistance: Replica Plating
65 Polymerase Chain Reaction for Amplifying DNA
66 Bacterial Transformation
Part 13 Medical Microbiology
67 The Staphylococci: Isolation and Identification
68 The Streptococci and Enterococci: Isolation and Identification
69 Gram-Negative Intestinal Pathogens
70 A Synthetic Epidemic
Part 14 Immunology and Serology
71 Slide Agglutination Test: Serological Typing
72 Slide Agglutination Test for S. aureus
73 Slide Agglutination Test for Streptococcus
74 Enzyme-Linked Immunosorbent Assay (ELISA)
75 Blood Grouping
Appendices
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