Benson's Microbiological Applications Laboratory Manual--Complete Version https://www.mheducation.com/cover-images/Jpeg_400-high/125991979X.jpeg 14 9781259919794 Benson's Microbiological Applications has been the "gold standard" of microbiology laboratory manuals for over 35 years. This manual has a number of attractive features that resulted in its adoption in universities, colleges, and community colleges. These features include user-friendly diagrams that students can easily follow, clear instructions, and an excellent array of reliable exercises suitable for beginning or advanced microbiology courses. In revising the lab manual for the fourteenth edition, we have tried to maintain the proven strengths of the manual and further enhance it. We have updated the introductory material of the fungi, protozoa, and algae to reflect changes in scientific information. Finally, the names of microorganisms used by the American Type Culture Collection. This is important for those users who rely on the ATCC for a source of cultures.
Benson's Microbiological Applications Laboratory Manual--Complete Version

Benson's Microbiological Applications Laboratory Manual--Complete Version

14th Edition
By Alfred Brown and Heidi Smith
ISBN10: 125991979X
ISBN13: 9781259919794
Copyright: 2017
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09781259919794

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ISBN10: 125991979X | ISBN13: 9781259919794

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Program Details

Part 1 Microscopy

1 Brightfield Microscopy

2 Darkfield Microscopy

3 Phase-Contrast Microscopy

4 Fluorescence Microscopy

5 Microscopic Measurements

Part 2 Survey of Microorganisms

6 Microbiology of Pond Water - Protists, Algae, and Cyanobacteria

7 Ubiquity of Bacteria

8 The Fungi: Molds and Yeasts

Part 3 Manipulation of Microorganisms

9 Aseptic Technique

10 Pure Culture Techniques

Part 4 Staining and Observations of Microorganisms

11 Smear Preparation

12 Simple Staining

13 Negative Staining

14 Capsular Staining

15 Gram Staining

16 Spore Staining: Two Methods

17 Acid-Fast Staining: Kinyoun Method

18 Motility Determination

Part 5 Culture Methods

19 Culture Media Preparation

20 Preparation of Stock Cultures

21 Enumeration of Bacteria: The Standard Plate Count

22 Slide Culture: Fungi

Part 6 Bacterial Viruses

23 Determination of a Bacteriophage Titer

24 A One-Step Bacteriophage Growth Curve

25 Isolation of Phage from Flies

26 Phage Typing

Part 7 Environmental Influences and Control of Microbial Growth

27 Effects of Oxygen on Growth

28 Temperature: Effects on Growth

29 pH and Microbial Growth

30 Water Activity and Osmotic Pressure

31 Ultraviolet Light: Lethal Effects

32 The Effects of Lysozyme on Bacterial Cells

33 Evaluation of Alcohol: Its Effectiveness as an Antiseptic

34 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method

35 Evaluation of Antiseptics: The Filter Paper Disk Method

36 Effectiveness of Hand Scrubbing

Part 8 Identification of Unknown Bacteria

37 Morphological Study of An Unknown Bacterium

38 Cultural Characteristics

39 Physiological Characteristics: Oxidation and Fermentation Stet Reactions

40 Physiological Characteristics: Hydrolytic and Degradative Stet Reactions

41 Physiological Characteristics: Multiple Test Media

42 Use of Bergey’s Manual

Part 9 Miniaturized Multitest Systems

43 Enterobacteriaceae Identification: The API 20E System

44 Enterobacteriaceae Identification: Entero-Pluri Test

45 Staphylococcus Identification: The API Staph System

Part 10 Diversity and Environmental Microbiology

46 Isolation of an Antibiotic Producer: The Streptomyces

47 Nitrogen Cycle: Ammonification

48 Symbiotic Nitrogen Fixation: Rhizobium

49 Free-Living Nitrogen Fixation: Azotobacter

50 Denitrification: Paracoccus denitrificans

51 The Winogradsky Column

52 Purple Nonsulfur Photosynthetic Bacteria

53 Sulfate Reducing Bacteria: Desulfovibrio

54 Bacterial Commensalism

55 Bacterial Synergism

56 Microbial Antagonism

Part 11 Applied Microbiology

57 Bacterial Food Counts

58 Bacteriological Examination of Water: Most Probable Number Determination

59 Bacteriological Examination of Water: The Membrane Filter Method

60 Reductase Test

61 Temperature: Lethal Effects

62 Microbial Spoilage of Canned Food

63 Microbiology of Alcohol Fermentation

Part 12 Bacterial Genetics and Biotechnology

64 Understanding Antibiotic Resistance: Replica Plating

65 Polymerase Chain Reaction for Amplifying DNA

66 Bacterial Transformation

Part 13 Medical Microbiology

67 The Staphylococci: Isolation and Identification

68 The Streptococci and Enterococci: Isolation and Identification

69 Gram-Negative Intestinal Pathogens

70 A Synthetic Epidemic

Part 14 Immunology and Serology

71 Slide Agglutination Test: Serological Typing

72 Slide Agglutination Test for S. aureus

73 Slide Agglutination Test for Streptococcus

74 Enzyme-Linked Immunosorbent Assay (ELISA)

75 Blood Grouping

Appendices

About the Author

Alfred Brown

Professor and Chair, Consultant Environmental Microbiology

Heidi Smith

Heidi Smith leads the microbiology discipline at Front Range Community College in Fort Collins, CO, teaching a variety of biology courses each semester including microbiology, anatomy/ physiology, and biotechnology Collaboration with other faculty across the nation, the development and implementation of new digital learning tools, and her focus on student learning outcomes have revolutionized her face-to-face and online teaching approaches and student performance in her classes. Outside of the classroom, Heidi oversees a federal grant program designed to train and support underrepresented students for undergraduate research and transfer into four-year STEM degree programs. She is also an active member of the American Society for Microbiology and participated as a task force member for the development of their Curriculum Guidelines for Undergraduate Microbiology Education. Off campus, Heidi enjoys the beautiful Colorado outdoors with her husband and three young children.

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