Benson's Microbiological Applications Laboratory Manual--Complete Version14^th Edition

By Alfred Brown and Heidi Smith
Copyright: 2017

Benson's Microbiological Applications Laboratory Manual--Complete Version

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Benson's Microbiological Applications Laboratory Manual--Complete Version14^th Edition

By Alfred Brown and Heidi Smith
Copyright: 2017

– Connect: A highly reliable, easy-to-use homework and learning management solution that embeds learning science and award-winning adaptive tools to improve student results.

- Benson's Microbiological Applications has been the "gold standard" of microbiology laboratory manuals for over 35 years.

- This manual features user-friendly diagrams that students can easily follow, clear instructions, and an excellent array of reliable exercises suitable for beginning or advanced microbiology courses.

- In revising the lab manual for the fourteenth edition, the authors worked hard to retain the proven strengths of the manual and while further enhancing it.

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Benson's Microbiological Applications Laboratory Manual--Complete Version14^th Edition

By Alfred Brown and Heidi Smith
Copyright: 2017

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ISBN10: 1259919706 | ISBN13: 9781259919701

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ISBN10: 125991979X | ISBN13: 9781259919794

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The estimated amount of time this product will be on the market is based on a number of factors, including faculty input to instructional design and the prior revision cycle and updates to academic research-which typically results in a revision cycle ranging from every two to four years for this product. Pricing subject to change at any time.

The estimated amount of time this product will be on the market is based on a number of factors, including faculty input to instructional design and the prior revision cycle and updates to academic research-which typically results in a revision cycle ranging from every two to four years for this product. Pricing subject to change at any time.

Program Details

Part 1 Microscopy

1 Brightfield Microscopy

2 Darkfield Microscopy

3 Phase-Contrast Microscopy

4 Fluorescence Microscopy

5 Microscopic Measurements

Part 2 Survey of Microorganisms

6 Microbiology of Pond Water - Protists, Algae, and Cyanobacteria

7 Ubiquity of Bacteria

8 The Fungi: Molds and Yeasts

Part 3 Manipulation of Microorganisms

9 Aseptic Technique

10 Pure Culture Techniques

Part 4 Staining and Observations of Microorganisms

11 Smear Preparation

12 Simple Staining

13 Negative Staining

14 Capsular Staining

15 Gram Staining

16 Spore Staining: Two Methods

17 Acid-Fast Staining: Kinyoun Method

18 Motility Determination

Part 5 Culture Methods

19 Culture Media Preparation

20 Preparation of Stock Cultures

21 Enumeration of Bacteria: The Standard Plate Count

22 Slide Culture: Fungi

Part 6 Bacterial Viruses

23 Determination of a Bacteriophage Titer

24 A One-Step Bacteriophage Growth Curve

25 Isolation of Phage from Flies

26 Phage Typing

Part 7 Environmental Influences and Control of Microbial Growth

27 Effects of Oxygen on Growth

28 Temperature: Effects on Growth

29 pH and Microbial Growth

30 Water Activity and Osmotic Pressure

31 Ultraviolet Light: Lethal Effects

32 The Effects of Lysozyme on Bacterial Cells

33 Evaluation of Alcohol: Its Effectiveness as an Antiseptic

34 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method

35 Evaluation of Antiseptics: The Filter Paper Disk Method

36 Effectiveness of Hand Scrubbing

Part 8 Identification of Unknown Bacteria

37 Morphological Study of An Unknown Bacterium

38 Cultural Characteristics

39 Physiological Characteristics: Oxidation and Fermentation Stet Reactions

40 Physiological Characteristics: Hydrolytic and Degradative Stet Reactions

41 Physiological Characteristics: Multiple Test Media

42 Use of Bergey’s Manual

Part 9 Miniaturized Multitest Systems

43 Enterobacteriaceae Identification: The API 20E System

44 Enterobacteriaceae Identification: Entero-Pluri Test

45 Staphylococcus Identification: The API Staph System

Part 10 Diversity and Environmental Microbiology

46 Isolation of an Antibiotic Producer: The Streptomyces

47 Nitrogen Cycle: Ammonification

48 Symbiotic Nitrogen Fixation: Rhizobium

49 Free-Living Nitrogen Fixation: Azotobacter

50 Denitrification: Paracoccus denitrificans

51 The Winogradsky Column

52 Purple Nonsulfur Photosynthetic Bacteria

53 Sulfate Reducing Bacteria: Desulfovibrio

54 Bacterial Commensalism

55 Bacterial Synergism

56 Microbial Antagonism

Part 11 Applied Microbiology

57 Bacterial Food Counts

58 Bacteriological Examination of Water: Most Probable Number Determination

59 Bacteriological Examination of Water: The Membrane Filter Method

60 Reductase Test

61 Temperature: Lethal Effects

62 Microbial Spoilage of Canned Food

63 Microbiology of Alcohol Fermentation

Part 12 Bacterial Genetics and Biotechnology

64 Understanding Antibiotic Resistance: Replica Plating

65 Polymerase Chain Reaction for Amplifying DNA

66 Bacterial Transformation

Part 13 Medical Microbiology

67 The Staphylococci: Isolation and Identification

68 The Streptococci and Enterococci: Isolation and Identification

69 Gram-Negative Intestinal Pathogens

70 A Synthetic Epidemic

Part 14 Immunology and Serology

71 Slide Agglutination Test: Serological Typing

72 Slide Agglutination Test for S. aureus

73 Slide Agglutination Test for Streptococcus

74 Enzyme-Linked Immunosorbent Assay (ELISA)

75 Blood Grouping

Appendices

TABLE OF CONTENTS

Part 1 Microscopy

1 Brightfield Microscopy

2 Darkfield Microscopy

3 Phase-Contrast Microscopy

4 Fluorescence Microscopy

5 Microscopic Measurements

Part 2 Survey of Microorganisms

6 Microbiology of Pond Water - Protists, Algae, and Cyanobacteria

7 Ubiquity of Bacteria

8 The Fungi: Molds and Yeasts

Part 3 Manipulation of Microorganisms

9 Aseptic Technique

10 Pure Culture Techniques

Part 4 Staining and Observations of Microorganisms

11 Smear Preparation

12 Simple Staining

13 Negative Staining

14 Capsular Staining

15 Gram Staining

16 Spore Staining: Two Methods

17 Acid-Fast Staining: Kinyoun Method

18 Motility Determination

Part 5 Culture Methods

19 Culture Media Preparation

20 Preparation of Stock Cultures

21 Enumeration of Bacteria: The Standard Plate Count

22 Slide Culture: Fungi

Part 6 Bacterial Viruses

23 Determination of a Bacteriophage Titer

24 A One-Step Bacteriophage Growth Curve

25 Isolation of Phage from Flies

26 Phage Typing

Part 7 Environmental Influences and Control of Microbial Growth

27 Effects of Oxygen on Growth

28 Temperature: Effects on Growth

29 pH and Microbial Growth

30 Water Activity and Osmotic Pressure

31 Ultraviolet Light: Lethal Effects

32 The Effects of Lysozyme on Bacterial Cells

33 Evaluation of Alcohol: Its Effectiveness as an Antiseptic

34 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method

35 Evaluation of Antiseptics: The Filter Paper Disk Method

36 Effectiveness of Hand Scrubbing

Part 8 Identification of Unknown Bacteria

37 Morphological Study of An Unknown Bacterium

38 Cultural Characteristics

39 Physiological Characteristics: Oxidation and Fermentation Stet Reactions

40 Physiological Characteristics: Hydrolytic and Degradative Stet Reactions

41 Physiological Characteristics: Multiple Test Media

42 Use of Bergey’s Manual

Part 9 Miniaturized Multitest Systems

43 Enterobacteriaceae Identification: The API 20E System

44 Enterobacteriaceae Identification: Entero-Pluri Test

45 Staphylococcus Identification: The API Staph System

Part 10 Diversity and Environmental Microbiology

46 Isolation of an Antibiotic Producer: The Streptomyces

47 Nitrogen Cycle: Ammonification

48 Symbiotic Nitrogen Fixation: Rhizobium

49 Free-Living Nitrogen Fixation: Azotobacter

50 Denitrification: Paracoccus denitrificans

51 The Winogradsky Column

52 Purple Nonsulfur Photosynthetic Bacteria

53 Sulfate Reducing Bacteria: Desulfovibrio

54 Bacterial Commensalism

55 Bacterial Synergism

56 Microbial Antagonism

Part 11 Applied Microbiology

57 Bacterial Food Counts

58 Bacteriological Examination of Water: Most Probable Number Determination

59 Bacteriological Examination of Water: The Membrane Filter Method

60 Reductase Test

61 Temperature: Lethal Effects

62 Microbial Spoilage of Canned Food

63 Microbiology of Alcohol Fermentation

Part 12 Bacterial Genetics and Biotechnology

64 Understanding Antibiotic Resistance: Replica Plating

65 Polymerase Chain Reaction for Amplifying DNA

66 Bacterial Transformation

Part 13 Medical Microbiology

67 The Staphylococci: Isolation and Identification

68 The Streptococci and Enterococci: Isolation and Identification

69 Gram-Negative Intestinal Pathogens

70 A Synthetic Epidemic

Part 14 Immunology and Serology

71 Slide Agglutination Test: Serological Typing

72 Slide Agglutination Test for S. aureus

73 Slide Agglutination Test for Streptococcus

74 Enzyme-Linked Immunosorbent Assay (ELISA)

75 Blood Grouping

Appendices

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