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Table of Contents

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Program Details

Part 1 Microscopy

1 Brightfield Microscopy

2 Darkfield Microscopy

3 Phase-Contrast Microscopy

4 Fluorescence Microscopy

5 Microscopic Measurements

Part 2 Survey of Microorganisms

6 Microbiology of Pond Water - Protists, Algae, and Cyanobacteria

7 Ubiquity of Bacteria

8 The Fungi: Molds and Yeasts

Part 3 Manipulation of Microorganisms

9 Aseptic Technique

10 Pure Culture Techniques

Part 4 Staining and Observations of Microorganisms

11 Smear Preparation

12 Simple Staining

13 Negative Staining

14 Capsular Staining

15 Gram Staining

16 Spore Staining: Two Methods

17 Acid-Fast Staining: Kinyoun Method

18 Motility Determination

Part 5 Culture Methods

19 Culture Media Preparation

20 Preparation of Stock Cultures

21 Enumeration of Bacteria: The Standard Plate Count

22 Slide Culture: Fungi

Part 6 Bacterial Viruses

23 Determination of a Bacteriophage Titer

24 A One-Step Bacteriophage Growth Curve

25 Isolation of Phage from Flies

26 Phage Typing

Part 7 Environmental Influences and Control of Microbial Growth

27 Effects of Oxygen on Growth

28 Temperature: Effects on Growth

29 pH and Microbial Growth

30 Water Activity and Osmotic Pressure

31 Ultraviolet Light: Lethal Effects

32 The Effects of Lysozyme on Bacterial Cells

33 Evaluation of Alcohol: Its Effectiveness as an Antiseptic

34 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method

35 Evaluation of Antiseptics: The Filter Paper Disk Method

36 Effectiveness of Hand Scrubbing

Part 8 Identification of Unknown Bacteria

37 Morphological Study of An Unknown Bacterium

38 Cultural Characteristics

39 Physiological Characteristics: Oxidation and Fermentation Stet Reactions

40 Physiological Characteristics: Hydrolytic and Degradative Stet Reactions

41 Physiological Characteristics: Multiple Test Media

42 Use of Bergey’s Manual

Part 9 Miniaturized Multitest Systems

43 Enterobacteriaceae Identification: The API 20E System

44 Enterobacteriaceae Identification: Entero-Pluri Test

45 Staphylococcus Identification: The API Staph System

Part 10 Diversity and Environmental Microbiology

46 Isolation of an Antibiotic Producer: The Streptomyces

47 Nitrogen Cycle: Ammonification

48 Symbiotic Nitrogen Fixation: Rhizobium

49 Free-Living Nitrogen Fixation: Azotobacter

50 Denitrification: Paracoccus denitrificans

51 The Winogradsky Column

52 Purple Nonsulfur Photosynthetic Bacteria

53 Sulfate Reducing Bacteria: Desulfovibrio

54 Bacterial Commensalism

55 Bacterial Synergism

56 Microbial Antagonism

Part 11 Applied Microbiology

57 Bacterial Food Counts

58 Bacteriological Examination of Water: Most Probable Number Determination

59 Bacteriological Examination of Water: The Membrane Filter Method

60 Reductase Test

61 Temperature: Lethal Effects

62 Microbial Spoilage of Canned Food

63 Microbiology of Alcohol Fermentation

Part 12 Bacterial Genetics and Biotechnology

64 Understanding Antibiotic Resistance: Replica Plating

65 Polymerase Chain Reaction for Amplifying DNA

66 Bacterial Transformation

Part 13 Medical Microbiology

67 The Staphylococci: Isolation and Identification

68 The Streptococci and Enterococci: Isolation and Identification

69 Gram-Negative Intestinal Pathogens

70 A Synthetic Epidemic

Part 14 Immunology and Serology

71 Slide Agglutination Test: Serological Typing

72 Slide Agglutination Test for S. aureus

73 Slide Agglutination Test for Streptococcus

74 Enzyme-Linked Immunosorbent Assay (ELISA)

75 Blood Grouping

Appendices